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bt474 kd  (Synthego Inc)

 
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    Synthego Inc bt474 kd
    Merlin‐deficient breast cancer cells display a dysfunctional antioxidant system. Expression levels of GCLC and GCLM were decreased in MCF7 KD (A and B, respectively) ( P < 0.0001 and P = 0.0029, respectively) and T47D KD (C and D, respectively) ( P = 0.0001 and P < 0.0001, respectively) compared to their NT controls. In contrast, expression levels of GCLC and GCLM were increased in SUM159 Merlin (E and F, respectively) ( P = 0.0071 and P = 0.0027, respectively) compared to SUM159 VEC. Nrf2 activity was measured by an NQO1‐driven Nrf2 luciferase assay which showed decreased activity in MCF7 KD (G) ( P = 0.0111) and T47D KD (H) ( P = 0.0001) compared to their respective NT controls; Nrf2 activity was increased in (I) SUM159 Merlin ( P = 0.0095) compared to SUM159 VEC ( n = 3 for each group). (J) Protein levels of Nrf2 were decreased while Keap1 increased in Merlin‐deficient cells compared to controls; the opposite was seen in the comparison between SUM159 Merlin and SUM159 VEC ( n = 3 for each group). (K) SOD1 protein expression is decreased in NF2 ‐silenced breast cancer cells, MCF7 KD, T47D KD, and <t>BT474</t> KD compared to their non‐target (NT) controls. In contrast, SOD1 is upregulated in SUM159 Merlin compared to SUM159 VEC. Error bars represent ± SEM. Student's t ‐test was applied for statistical analysis. Band densitometry of immunoblotting is shown. β‐actin was used as loading control.
    Bt474 Kd, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bt474+kd/bt474+kd/pmc08024723-33-23-13
    Average 90 stars, based on 1 article reviews
    bt474 kd - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Merlin deficiency alters the redox management program in breast cancer"

    Article Title: Merlin deficiency alters the redox management program in breast cancer

    Journal: Molecular Oncology

    doi: 10.1002/1878-0261.12896

    Merlin‐deficient breast cancer cells display a dysfunctional antioxidant system. Expression levels of GCLC and GCLM were decreased in MCF7 KD (A and B, respectively) ( P < 0.0001 and P = 0.0029, respectively) and T47D KD (C and D, respectively) ( P = 0.0001 and P < 0.0001, respectively) compared to their NT controls. In contrast, expression levels of GCLC and GCLM were increased in SUM159 Merlin (E and F, respectively) ( P = 0.0071 and P = 0.0027, respectively) compared to SUM159 VEC. Nrf2 activity was measured by an NQO1‐driven Nrf2 luciferase assay which showed decreased activity in MCF7 KD (G) ( P = 0.0111) and T47D KD (H) ( P = 0.0001) compared to their respective NT controls; Nrf2 activity was increased in (I) SUM159 Merlin ( P = 0.0095) compared to SUM159 VEC ( n = 3 for each group). (J) Protein levels of Nrf2 were decreased while Keap1 increased in Merlin‐deficient cells compared to controls; the opposite was seen in the comparison between SUM159 Merlin and SUM159 VEC ( n = 3 for each group). (K) SOD1 protein expression is decreased in NF2 ‐silenced breast cancer cells, MCF7 KD, T47D KD, and BT474 KD compared to their non‐target (NT) controls. In contrast, SOD1 is upregulated in SUM159 Merlin compared to SUM159 VEC. Error bars represent ± SEM. Student's t ‐test was applied for statistical analysis. Band densitometry of immunoblotting is shown. β‐actin was used as loading control.
    Figure Legend Snippet: Merlin‐deficient breast cancer cells display a dysfunctional antioxidant system. Expression levels of GCLC and GCLM were decreased in MCF7 KD (A and B, respectively) ( P < 0.0001 and P = 0.0029, respectively) and T47D KD (C and D, respectively) ( P = 0.0001 and P < 0.0001, respectively) compared to their NT controls. In contrast, expression levels of GCLC and GCLM were increased in SUM159 Merlin (E and F, respectively) ( P = 0.0071 and P = 0.0027, respectively) compared to SUM159 VEC. Nrf2 activity was measured by an NQO1‐driven Nrf2 luciferase assay which showed decreased activity in MCF7 KD (G) ( P = 0.0111) and T47D KD (H) ( P = 0.0001) compared to their respective NT controls; Nrf2 activity was increased in (I) SUM159 Merlin ( P = 0.0095) compared to SUM159 VEC ( n = 3 for each group). (J) Protein levels of Nrf2 were decreased while Keap1 increased in Merlin‐deficient cells compared to controls; the opposite was seen in the comparison between SUM159 Merlin and SUM159 VEC ( n = 3 for each group). (K) SOD1 protein expression is decreased in NF2 ‐silenced breast cancer cells, MCF7 KD, T47D KD, and BT474 KD compared to their non‐target (NT) controls. In contrast, SOD1 is upregulated in SUM159 Merlin compared to SUM159 VEC. Error bars represent ± SEM. Student's t ‐test was applied for statistical analysis. Band densitometry of immunoblotting is shown. β‐actin was used as loading control.

    Techniques Used: Expressing, Activity Assay, Luciferase, Western Blot

    Related Articles

    Expressing:

    Article Title: Merlin deficiency alters the redox management program in breast cancer
    Article Snippet: transducing pLV‐CMV‐NF2‐GFP‐2A‐Puro lentiviral particles (Capital Biosciences, Gaithersburg, MD, USA) and cultured as previously described [ , ]. .. The cell lines T47D and BT474 knocked out for NF2 were acquired from Synthego, Redwood City, CA, USA and named T47D KD and BT474 KD, respectively. .. The T47D pair was cultured in RPMI 1640 medium (Thermo Fisher, Grand Island, NY, USA) + 10% heat‐inactivated FBS (Thermo Fisher) + 10 µg·mL −1 insulin

    Activity Assay:

    Article Title: Merlin deficiency alters the redox management program in breast cancer
    Article Snippet: transducing pLV‐CMV‐NF2‐GFP‐2A‐Puro lentiviral particles (Capital Biosciences, Gaithersburg, MD, USA) and cultured as previously described [ , ]. .. The cell lines T47D and BT474 knocked out for NF2 were acquired from Synthego, Redwood City, CA, USA and named T47D KD and BT474 KD, respectively. .. The T47D pair was cultured in RPMI 1640 medium (Thermo Fisher, Grand Island, NY, USA) + 10% heat‐inactivated FBS (Thermo Fisher) + 10 µg·mL −1 insulin

    Luciferase:

    Article Title: Merlin deficiency alters the redox management program in breast cancer
    Article Snippet: transducing pLV‐CMV‐NF2‐GFP‐2A‐Puro lentiviral particles (Capital Biosciences, Gaithersburg, MD, USA) and cultured as previously described [ , ]. .. The cell lines T47D and BT474 knocked out for NF2 were acquired from Synthego, Redwood City, CA, USA and named T47D KD and BT474 KD, respectively. .. The T47D pair was cultured in RPMI 1640 medium (Thermo Fisher, Grand Island, NY, USA) + 10% heat‐inactivated FBS (Thermo Fisher) + 10 µg·mL −1 insulin

    Western Blot:

    Article Title: Merlin deficiency alters the redox management program in breast cancer
    Article Snippet: transducing pLV‐CMV‐NF2‐GFP‐2A‐Puro lentiviral particles (Capital Biosciences, Gaithersburg, MD, USA) and cultured as previously described [ , ]. .. The cell lines T47D and BT474 knocked out for NF2 were acquired from Synthego, Redwood City, CA, USA and named T47D KD and BT474 KD, respectively. .. The T47D pair was cultured in RPMI 1640 medium (Thermo Fisher, Grand Island, NY, USA) + 10% heat‐inactivated FBS (Thermo Fisher) + 10 µg·mL −1 insulin



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    bt474 kd  (Synthego Inc)
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    Synthego Inc bt474 kd
    Merlin‐deficient breast cancer cells display a dysfunctional antioxidant system. Expression levels of GCLC and GCLM were decreased in MCF7 KD (A and B, respectively) ( P < 0.0001 and P = 0.0029, respectively) and T47D KD (C and D, respectively) ( P = 0.0001 and P < 0.0001, respectively) compared to their NT controls. In contrast, expression levels of GCLC and GCLM were increased in SUM159 Merlin (E and F, respectively) ( P = 0.0071 and P = 0.0027, respectively) compared to SUM159 VEC. Nrf2 activity was measured by an NQO1‐driven Nrf2 luciferase assay which showed decreased activity in MCF7 KD (G) ( P = 0.0111) and T47D KD (H) ( P = 0.0001) compared to their respective NT controls; Nrf2 activity was increased in (I) SUM159 Merlin ( P = 0.0095) compared to SUM159 VEC ( n = 3 for each group). (J) Protein levels of Nrf2 were decreased while Keap1 increased in Merlin‐deficient cells compared to controls; the opposite was seen in the comparison between SUM159 Merlin and SUM159 VEC ( n = 3 for each group). (K) SOD1 protein expression is decreased in NF2 ‐silenced breast cancer cells, MCF7 KD, T47D KD, and <t>BT474</t> KD compared to their non‐target (NT) controls. In contrast, SOD1 is upregulated in SUM159 Merlin compared to SUM159 VEC. Error bars represent ± SEM. Student's t ‐test was applied for statistical analysis. Band densitometry of immunoblotting is shown. β‐actin was used as loading control.
    Bt474 Kd, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bt474+kd/bt474+kd/pmc08024723-33-23-13
    Average 90 stars, based on 1 article reviews
    bt474 kd - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Merlin‐deficient breast cancer cells display a dysfunctional antioxidant system. Expression levels of GCLC and GCLM were decreased in MCF7 KD (A and B, respectively) ( P < 0.0001 and P = 0.0029, respectively) and T47D KD (C and D, respectively) ( P = 0.0001 and P < 0.0001, respectively) compared to their NT controls. In contrast, expression levels of GCLC and GCLM were increased in SUM159 Merlin (E and F, respectively) ( P = 0.0071 and P = 0.0027, respectively) compared to SUM159 VEC. Nrf2 activity was measured by an NQO1‐driven Nrf2 luciferase assay which showed decreased activity in MCF7 KD (G) ( P = 0.0111) and T47D KD (H) ( P = 0.0001) compared to their respective NT controls; Nrf2 activity was increased in (I) SUM159 Merlin ( P = 0.0095) compared to SUM159 VEC ( n = 3 for each group). (J) Protein levels of Nrf2 were decreased while Keap1 increased in Merlin‐deficient cells compared to controls; the opposite was seen in the comparison between SUM159 Merlin and SUM159 VEC ( n = 3 for each group). (K) SOD1 protein expression is decreased in NF2 ‐silenced breast cancer cells, MCF7 KD, T47D KD, and BT474 KD compared to their non‐target (NT) controls. In contrast, SOD1 is upregulated in SUM159 Merlin compared to SUM159 VEC. Error bars represent ± SEM. Student's t ‐test was applied for statistical analysis. Band densitometry of immunoblotting is shown. β‐actin was used as loading control.

    Journal: Molecular Oncology

    Article Title: Merlin deficiency alters the redox management program in breast cancer

    doi: 10.1002/1878-0261.12896

    Figure Lengend Snippet: Merlin‐deficient breast cancer cells display a dysfunctional antioxidant system. Expression levels of GCLC and GCLM were decreased in MCF7 KD (A and B, respectively) ( P < 0.0001 and P = 0.0029, respectively) and T47D KD (C and D, respectively) ( P = 0.0001 and P < 0.0001, respectively) compared to their NT controls. In contrast, expression levels of GCLC and GCLM were increased in SUM159 Merlin (E and F, respectively) ( P = 0.0071 and P = 0.0027, respectively) compared to SUM159 VEC. Nrf2 activity was measured by an NQO1‐driven Nrf2 luciferase assay which showed decreased activity in MCF7 KD (G) ( P = 0.0111) and T47D KD (H) ( P = 0.0001) compared to their respective NT controls; Nrf2 activity was increased in (I) SUM159 Merlin ( P = 0.0095) compared to SUM159 VEC ( n = 3 for each group). (J) Protein levels of Nrf2 were decreased while Keap1 increased in Merlin‐deficient cells compared to controls; the opposite was seen in the comparison between SUM159 Merlin and SUM159 VEC ( n = 3 for each group). (K) SOD1 protein expression is decreased in NF2 ‐silenced breast cancer cells, MCF7 KD, T47D KD, and BT474 KD compared to their non‐target (NT) controls. In contrast, SOD1 is upregulated in SUM159 Merlin compared to SUM159 VEC. Error bars represent ± SEM. Student's t ‐test was applied for statistical analysis. Band densitometry of immunoblotting is shown. β‐actin was used as loading control.

    Article Snippet: The cell lines T47D and BT474 knocked out for NF2 were acquired from Synthego, Redwood City, CA, USA and named T47D KD and BT474 KD, respectively.

    Techniques: Expressing, Activity Assay, Luciferase, Western Blot